Publications

Link to T Duchaine in PubMed.

2024

EDC-3 and EDC-4 Regulate Embryonic mRNA Clearance and Biomolecular Condensate Specialization.
Vidya E, Jami-Alahmadi Y, Mayank AK, Rizwan J, Stella Xu JMS, Cheng T, Leventis R, Sonenberg N, Wohlschlegel JA, Vera M, Duchaine TF. Cell Rep. 2024. https://doi.org/10.1016/j.celrep.2024.114781

Paraspeckle-Independent Co-Transcriptional Regulation of Nuclear MicroRNA Biogenesis by SFPQ.
Thivierge C, Bellefeuille M, Diwan SS, Dyakov BJA, Leventis R, Perron G, Najafabadi HS, Gravel SP, Gingras AC, Duchaine TF. Cell Rep. 2024. https://doi.org/10.1016/j.celrep.2024.114695

Repression of mRNA translation initiation by GIGYF1 via disrupting the eIF3-eIF4G1 interaction.
Choi JH, Luo J, Hesketh GG, Guo S, Pistofidis A, Ladak RJ, An Y, Naeli P, Alain T, Schmeing TM, Gingras AC, Duchaine T, Zhang X, Sonenberg N, Jafarnejad SM. Sci Advances. 2024. https://doi.org/10.1126/sciadv.adl5638

2023

The SARS-CoV-2 protein NSP2 enhances microRNA-mediated translational repression.
Naeli P, Zhang X, Snell PH, Chatterjee S, Kamran M, Ladak RJ, Orr N, Duchaine T, Sonenberg N, Jafarnejad SM. J Cell Sci. 2023. https://doi.org/10.1242/jcs.261286

2022

SARS-CoV-2 impairs interferon production via NSP2-induced repression of mRNA translation.
Xu Z, Choi JH, Dai DL, Luo J, Ladak RJ, Li Q, Wang Y, Zhang C, Wiebe S, Liu ACH, Ran X, Yang J, Naeli P, Garzia A, Zhou L, Mahmood N, Deng Q, Elaish M, Lin R, Mahal LK, Hobman TC, Pelletier J, Alain T, Vidal SM, Duchaine T, Mazhab-Jafari MT, Mao X, Jafarnejad SM, Sonenberg N. Proc Natl Acad Sci U S A. 2022. https://doi.org/10.1073/pnas.2204539119

Distinct, opposing functions for CFIm59 and CFIm68 in mRNA alternative polyadenylation of Pten and in the PI3K/Akt signalling cascade. Tseng HW, Mota-Sydor A, Leventis R, Jovanovic P, Topisirovic I, Duchaine TF. Nucleic Acids Res. 2022. https://doi.org/10.1093/nar/gkac704

Eukaryotic mRNA Decapping Activation.
Vidya E, Duchaine TF. Front Genet. 2022. https://doi.org/10.3389/fgene.2022.832547

2021

Novel LOTUS-domain proteins are organizational hubs that recruit C. elegans Vasa to germ granules.
Cipriani PG, Bay O, Zinno J, Gutwein M, Gan HH, Mayya VK, Chung G, Chen JX, Fahs H, Guan Y, Duchaine TF, Selbach M, Piano F, Gunsalus KC. Elife. 2021. https://doi.org/10.7554/elife.60833

microRNA-mediated translation repression through GYF-1 and IFE-4 in C. elegans development.
Mayya VK, Flamand MN, Lambert AM, Jafarnejad SM, Wohlschlegel JA, Sonenberg N, Duchaine TF. Nucleic Acids Res. 2021. https://doi.org/10.1093/nar/gkab162

2020

Naive Human Embryonic Stem Cells Can Give Rise to Cells with a Trophoblast-like Transcriptome and Methylome.
Cinkornpumin JK, Kwon SY, Guo Y, Hossain I, Sirois J, Russett CS, Tseng HW, Okae H, Arima T, Duchaine TF, Liu W, Pastor WA. Stem Cell Reports. 2020. https://doi.org/10.1016/j.stemcr.2020.06.003

A Family of Argonaute-Interacting Proteins Gates Nuclear RNAi.
Lewis A, Berkyurek AC, Greiner A, Sawh AN, Vashisht A, Merrett S, Flamand MN, Wohlschlegel J, Sarov M, Miska EA, Duchaine TF. Mol Cell. 2020. https://doi.org/10.1016/j.molcel.2020.04.007

Repression of LKB1 by miR-17∼92 Sensitizes MYC-Dependent Lymphoma to Biguanide Treatment.
Izreig S, Gariepy A, Kaymak I, Bridges HR, Donayo AO, Bridon G, DeCamp LM, Kitchen-Goosen SM, Avizonis D, Sheldon RD, Laister RC, Minden MD, Johnson NA, Duchaine TF, Rudoltz MS, Yoo S, Pollak MN, Williams KS, Jones RG. Cell Rep Med. 2020. https://doi.org/10.1016/j.xcrm.2020.100014

2019

MiR-35 buffers apoptosis thresholds in the C. elegans germline by antagonizing both MAPK and core apoptosis pathways.
Tran AT, Chapman EM, Flamand MN, Yu B, Krempel SJ, Duchaine TF, Eroglu M, Derry WB. Cell Death Differ. 2019. https://doi.org/10.1038/s41418-019-0325-6

Oncogenic Biogenesis of pri-miR-17∼92 Reveals Hierarchy and Competition among Polycistronic MicroRNAs.
Donayo AO, Johnson RM, Tseng HW, Izreig S, Gariepy A, Mayya VK, Wu E, Alam R, Lussier C, Jones RG, Duchaine TF. Mol Cell. 2019. https://doi.org/10.1016/j.molcel.2019.05.033

Mechanistic Insights into MicroRNA-Mediated Gene Silencing.
Duchaine TF, Fabian MR. Cold Spring Harb Perspect Biol. 2019. https://doi.org/10.1101/cshperspect.a032771

Ciphers and Executioners: How 3'-Untranslated Regions Determine the Fate of Messenger RNAs.
Mayya VK, Duchaine TF. Front Genet. 2019. https://doi.org/10.3389/fgene.2019.00006

2018

Alternative polyadenylation confers Pten mRNAs stability and resistance to microRNAs.
Thivierge C, Tseng HW, Mayya VK, Lussier C, Gravel SP, Duchaine TF. Nucleic Acids Res. 2018. https://doi.org/10.1093/nar/gky666

Translational control of ERK signaling through miRNA/4EHP-directed silencing.
Jafarnejad SM, Chapat C, Matta-Camacho E, Gelbart IA, Hesketh GG, Arguello M, Garzia A, Kim SH, Attig J, Shapiro M, Morita M, Khoutorsky A, Alain T, Gkogkas CG, Stern-Ginossar N, Tuschl T, Gingras AC, Duchaine TF, Sonenberg N. Elife. 2018. https://doi.org/10.7554/elife.35034

2017

Cap-binding protein 4EHP effects translation silencing by microRNAs.

Chapat C, Jafarnejad SM, Matta-Camacho E, Hesketh GG, Gelbart IA, Attig J, Gkogkas CG, Alain T, Stern-Ginossar N, Fabian MR, Gingras AC, Duchaine TF, Sonenberg N. PNAS. https://doi.org/10.1073/pnas.1701488114

A non-canonical site reveals the cooperative mechanisms of microRNA-mediated silencing.

Flamand MN, Gan HH, Mayya VK, Gunsalus KC, Duchaine TF. Nucleic Acids Research. https://doi.org/10.1093/nar/gkx340

A continuum of mRNP complexes in embryonic microRNA-mediated silencing.

Wu E, Vashisht A, Chapat C, Flamand MN, Cohen E, Sarov M, Tabach Y, Sonenberg N, Wohlschlegel J, Duchaine TF. Nucleic Acids Research. https://doi.org/10.1093/nar/gkw872

2016

The miR-17∼92 microRNA Cluster Is a Global Regulator of Tumor Metabolism.
Izreig S, Samborska B, Johnson RM, Sergushichev A, Ma EH, Lussier C, Loginicheva E, Donayo AO, Poffenberger MC, Sagan SM, Vincent EE, Artyomov MN, Duchaine TF, Jones RG. Cell Rep. 2016. https://doi.org/10.1016/j.celrep.2016.07.036

Poly(A)-binding proteins are required for microRNA-mediated silencing and to promote target deadenylation in C. elegans.
Flamand MN, Wu E, Vashisht A, Jannot G, Keiper BD, Simard MJ, Wohlschlegel J, Duchaine TF. Nucleic Acids Res. 2016. https://doi.org/10.1093/nar/gkw276

2015

On the availability of microRNA-induced silencing complexes, saturation of microRNA-binding sites and stoichiometry.
Mayya VK, Duchaine TF. Nucleic Acids Res. 2015. https://doi.org/10.1093/nar/gkv720

FLCN and AMPK Confer Resistance to Hyperosmotic Stress via Remodeling of Glycogen Stores.
Possik E, Ajisebutu A, Manteghi S, Gingras MC, Vijayaraghavan T, Flamand M, Coull B, Schmeisser K, Duchaine T, van Steensel M, Hall DH, Pause A. PLoS Genet. 2015. https://doi.org/10.1371/journal.pgen.1005520

2014

DICER1: mutations, microRNAs and mechanisms.
Foulkes WD, Priest JR, Duchaine TF. Nat Rev Cancer. 2014. https://doi.org/10.1038/nrc3802

Human DDX6 effects miRNA-mediated gene silencing via direct binding to CNOT1.Rouya C, Siddiqui N, Morita M, Duchaine TF, Fabian MR, Sonenberg N. RNA. 2014. https://doi.org/10.1261/rna.045302.114

Fusion of TTYH1 with the C19MC microRNA cluster drives expression of a brain-specific DNMT3B isoform in the embryonal brain tumor ETMR. Kleinman CL, Gerges N, Papillon-Cavanagh S, Sin-Chan P, Pramatarova A, Quang DA, Adoue V, Busche S, Caron M, Djambazian H, Bemmo A, Fontebasso AM, Spence T, Schwartzentruber J, Albrecht S, Hauser P, Garami M, Klekner A, Bognar L, Montes JL, Staffa A, Montpetit A, Berube P, Zakrzewska M, Zakrzewski K, Liberski PP, Dong Z, Siegel PM, Duchaine T, Perotti C, Fleming A, Faury D, Remke M, Gallo M, Dirks P, Taylor MD, Sladek R, Pastinen T, Chan JA, Huang A, Majewski J, Jabado N. Nat Genet. 2014. https://doi.org/10.1038/ng.2849

2013

A truncated form of dicer tilts the balance of RNA interference pathways.
Sawh AN, Duchaine TF. Cell Rep. 2013. https://doi.org/10.1016/j.celrep.2013.07.013

2012

SnapShot: endogenous RNAi machinery and mechanisms. Flamand M, Duchaine TF. Cell. 2012. https://doi.org/10.1016/j.cell.2012.07.016

Snapshot: endogenous RNAi [corrected] pathways. Flamand M, Duchaine TF. Cell. 2012. https://doi.org/10.1016/j.cell.2012.07.006

Turning Dicer on its head. Sawh AN, Duchaine TF. Nat Struct Mol Biol. 2012. https://doi.org/10.1038/nsmb.2275

2011

Tudor domain ERI-5 tethers an RNA-dependent RNA polymerase to DCR-1 to potentiate endo-RNAi.
Thivierge C, Makil N, Flamand M, Vasale JJ, Mello CC, Wohlschlegel J, Conte D Jr, Duchaine TF. Nat Struct Mol Biol. 2011. https://doi.org/10.1038/nsmb.2186

miRNA-mediated deadenylation is orchestrated by GW182 through two conserved motifs that interact with CCR4-NOT.
Fabian MR, Cieplak MK, Frank F, Morita M, Green J, Srikumar T, Nagar B, Yamamoto T, Raught B, Duchaine TF, Sonenberg N. Nat Struct Mol Biol. 2011. https://doi.org/10.1038/nsmb.2149

Cell-free microRNA-mediated translation repression in Caenorhabditis elegans.
Wu E, Duchaine TF. Methods Mol Biol. 2011. https://doi.org/10.1007/978-1-61779-046-1_15

2010

Pervasive and cooperative deadenylation of 3'UTRs by embryonic microRNA families.
Wu E, Thivierge C, Flamand M, Mathonnet G, Vashisht AA, Wohlschlegel J, Fabian MR, Sonenberg N, Duchaine TF. Mol Cell. 2010. https://doi.org/10.1016/j.molcel.2010.11.003

Dicer's helicase domain is required for accumulation of some, but not all, C. elegans endogenous siRNAs.
Welker NC, Pavelec DM, Nix DA, Duchaine TF, Kennedy S, Bass BL. RNA. 2010. https://doi.org/10.1261/rna.2122010

Multimerization of Staufen1 in live cells.
C, Dugré-Brisson S, Boulay K, Breton B, Lapointe G, Armando S, Trépanier V, Duchaîne T, Bouvier M, Desgroseillers L. RNA. 2010. http://www.rnajournal.org/cgi/doi/10.1261/rna.1664210

Sequential rounds of RNA-dependent RNA transcription drive endogenous small-RNA biogenesis in the ERGO-1/Argonaute pathway. Vasale JJ, Gu W, Thivierge C, Batista PJ, Claycomb JM, Youngman EM, Duchaine TF, Mello CC, Conte D Jr. Proc Natl Acad Sci U S A. 2010. https://doi.org/10.1073/pnas.0911908107

2009

Requirement for the ERI/DICER complex in endogenous RNA interference and sperm development in Caenorhabditis elegans. Pavelec DM, Lachowiec J, Duchaine TF, Smith HE, Kennedy S. Genetics. 2009. https://doi.org/10.1534/genetics.109.108134

Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation.
Fabian MR, Mathonnet G, Sundermeier T, Mathys H, Zipprich JT, Svitkin YV, Rivas F, Jinek M, Wohlschlegel J, Doudna JA, Chen CY, Shyu AB, Yates JR 3rd, Hannon GJ, Filipowicz W, Duchaine TF, Sonenberg N. Mol Cell. 2009. https://doi.org/10.1016/j.molcel.2009.08.004

RNA interference and micro RNA-oriented therapy in cancer: rationales, promises, and challenges.
Duchaine TF, Slack FJ. Curr Oncol. 2009. https://doi.org/10.3747/co.v16i4.486

2007

MicroRNA inhibition of translation initiation in vitro by targeting the cap-binding complex eIF4F.
Mathonnet G, Fabian MR, Svitkin YV, Parsyan A, Huck L, Murata T, Biffo S, Merrick WC, Darzynkiewicz E, Pillai RS, Filipowicz W, Duchaine TF, Sonenberg N. Science. 2007. https://doi.org/10.1126/science.1146067

2006

Functional proteomics reveals the biochemical niche of C. elegans DCR-1 in multiple small-RNA-mediated pathways.
Duchaine TF, Wohlschlegel JA, Kennedy S, Bei Y, Conte D Jr, Pang K, Brownell DR, Harding S, Mitani S, Ruvkun G, Yates JR 3rd, Mello CC. Cell. 2006. https://doi.org/10.1016/j.cell.2005.11.036

2002

Staufen2 isoforms localize to the somatodendritic domain of neurons and interact with different organelles.
Duchaine TF, Hemraj I, Furic L, Deitinghoff A, Kiebler MA, DesGroseillers L. J Cell Sci. 2002. https://doi.org/10.1242/jcs.115.16.3285

Molecular mapping of the determinants involved in human Staufen-ribosome association.
Luo M, Duchaine TF, DesGroseillers L. Biochem J. 2002. https://doi.org/10.1042/bj20020263

2000

A novel murine Staufen isoform modulates the RNA content of Staufen complexes. Duchaîne T, Wang HJ, Luo M, Steinberg SV, Nabi IR, DesGroseillers L. Mol Cell Biol. 2000. https://doi.org/10.1128/mcb.20.15.5592-5601.2000

Expression of autocrine motility factor/phosphohexose isomerase in Cos7 cells. Lagana A, Duchaine T, Raz A, DesGroseillers L, Nabi IR. Biochem Biophys Res Commun. 2000. https://doi.org/10.1006/bbrc.2000.2904

1999

Mammalian staufen is a double-stranded-RNA- and tubulin-binding protein which localizes to the rough endoplasmic reticulum.Wickham L, Duchaîne T, Luo M, Nabi IR, DesGroseillers L. Mol Cell Biol. 1999. https://doi.org/10.1128/MCB.19.3.2220

1998

Cloning and functional expression of an Aplysia 5-HT receptor negatively coupled to adenylate cyclase. Angers A, Storozhuk MV, Duchaîne T, Castellucci VF, DesGroseillers L. J Neurosci. 1998. https://doi.org/10.1523/jneurosci.18-15-05586.1998